Which test automation tool uses Python?
Selenium is the leading Test Automation tool in market which have python langauge binding. Integrated with PyUnit or PyTest framwork is very easy to start your WebTest Automation using Python and Selenium.
Learn to setup first python test enviroment here
Other popular testing frameworks listed here https://www.einfochips.com/blog/most-widely-used-python-based-test-automation-frameworks/